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Frequency of FKBP5 intron 7 methylation positivity in adolescents with PTSD and trauma-unexposed controls. Methylation status was assessed qualitatively (methylation detected vs. not detected) using Sanger sequencing of bisulfite-converted <t>PCR</t> products targeting the FKBP5 intron 7 region. Methylation positivity was detected in 10 of 36 adolescents with PTSD (27.8%) and in 3 of 36 trauma-unexposed controls (8.3%). The panel reports the relative risk with its bias-corrected and accelerated bootstrap interval and the two-sided Fisher’s exact p value (RR = 3.33, 95% BCa CI [0.96, 12.40]; Haldane-Anscombe corrected RR = 3.00; Fisher’s exact p = .063). Because expected cell counts are small, the contrast reaches the conventional threshold under the uncorrected Pearson χ ² test ( p = .032) but not under Fisher’s exact, continuity-corrected, or bootstrap approaches; the difference is therefore presented as an effect-size estimate. Error bars denote 95% confidence intervals for the group proportions. Site-level frequencies for the seven CpG sites are given in Supplementary Table S7.
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pcr amplification adopted premix taq  (TaKaRa)
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Frequency of FKBP5 intron 7 methylation positivity in adolescents with PTSD and trauma-unexposed controls. Methylation status was assessed qualitatively (methylation detected vs. not detected) using Sanger sequencing of bisulfite-converted <t>PCR</t> products targeting the FKBP5 intron 7 region. Methylation positivity was detected in 10 of 36 adolescents with PTSD (27.8%) and in 3 of 36 trauma-unexposed controls (8.3%). The panel reports the relative risk with its bias-corrected and accelerated bootstrap interval and the two-sided Fisher’s exact p value (RR = 3.33, 95% BCa CI [0.96, 12.40]; Haldane-Anscombe corrected RR = 3.00; Fisher’s exact p = .063). Because expected cell counts are small, the contrast reaches the conventional threshold under the uncorrected Pearson χ ² test ( p = .032) but not under Fisher’s exact, continuity-corrected, or bootstrap approaches; the difference is therefore presented as an effect-size estimate. Error bars denote 95% confidence intervals for the group proportions. Site-level frequencies for the seven CpG sites are given in Supplementary Table S7.
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Frequency of FKBP5 intron 7 methylation positivity in adolescents with PTSD and trauma-unexposed controls. Methylation status was assessed qualitatively (methylation detected vs. not detected) using Sanger sequencing of bisulfite-converted <t>PCR</t> products targeting the FKBP5 intron 7 region. Methylation positivity was detected in 10 of 36 adolescents with PTSD (27.8%) and in 3 of 36 trauma-unexposed controls (8.3%). The panel reports the relative risk with its bias-corrected and accelerated bootstrap interval and the two-sided Fisher’s exact p value (RR = 3.33, 95% BCa CI [0.96, 12.40]; Haldane-Anscombe corrected RR = 3.00; Fisher’s exact p = .063). Because expected cell counts are small, the contrast reaches the conventional threshold under the uncorrected Pearson χ ² test ( p = .032) but not under Fisher’s exact, continuity-corrected, or bootstrap approaches; the difference is therefore presented as an effect-size estimate. Error bars denote 95% confidence intervals for the group proportions. Site-level frequencies for the seven CpG sites are given in Supplementary Table S7.
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Frequency of FKBP5 intron 7 methylation positivity in adolescents with PTSD and trauma-unexposed controls. Methylation status was assessed qualitatively (methylation detected vs. not detected) using Sanger sequencing of bisulfite-converted <t>PCR</t> products targeting the FKBP5 intron 7 region. Methylation positivity was detected in 10 of 36 adolescents with PTSD (27.8%) and in 3 of 36 trauma-unexposed controls (8.3%). The panel reports the relative risk with its bias-corrected and accelerated bootstrap interval and the two-sided Fisher’s exact p value (RR = 3.33, 95% BCa CI [0.96, 12.40]; Haldane-Anscombe corrected RR = 3.00; Fisher’s exact p = .063). Because expected cell counts are small, the contrast reaches the conventional threshold under the uncorrected Pearson χ ² test ( p = .032) but not under Fisher’s exact, continuity-corrected, or bootstrap approaches; the difference is therefore presented as an effect-size estimate. Error bars denote 95% confidence intervals for the group proportions. Site-level frequencies for the seven CpG sites are given in Supplementary Table S7.
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Frequency of FKBP5 intron 7 methylation positivity in adolescents with PTSD and trauma-unexposed controls. Methylation status was assessed qualitatively (methylation detected vs. not detected) using Sanger sequencing of bisulfite-converted <t>PCR</t> products targeting the FKBP5 intron 7 region. Methylation positivity was detected in 10 of 36 adolescents with PTSD (27.8%) and in 3 of 36 trauma-unexposed controls (8.3%). The panel reports the relative risk with its bias-corrected and accelerated bootstrap interval and the two-sided Fisher’s exact p value (RR = 3.33, 95% BCa CI [0.96, 12.40]; Haldane-Anscombe corrected RR = 3.00; Fisher’s exact p = .063). Because expected cell counts are small, the contrast reaches the conventional threshold under the uncorrected Pearson χ ² test ( p = .032) but not under Fisher’s exact, continuity-corrected, or bootstrap approaches; the difference is therefore presented as an effect-size estimate. Error bars denote 95% confidence intervals for the group proportions. Site-level frequencies for the seven CpG sites are given in Supplementary Table S7.
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Frequency of FKBP5 intron 7 methylation positivity in adolescents with PTSD and trauma-unexposed controls. Methylation status was assessed qualitatively (methylation detected vs. not detected) using Sanger sequencing of bisulfite-converted <t>PCR</t> products targeting the FKBP5 intron 7 region. Methylation positivity was detected in 10 of 36 adolescents with PTSD (27.8%) and in 3 of 36 trauma-unexposed controls (8.3%). The panel reports the relative risk with its bias-corrected and accelerated bootstrap interval and the two-sided Fisher’s exact p value (RR = 3.33, 95% BCa CI [0.96, 12.40]; Haldane-Anscombe corrected RR = 3.00; Fisher’s exact p = .063). Because expected cell counts are small, the contrast reaches the conventional threshold under the uncorrected Pearson χ ² test ( p = .032) but not under Fisher’s exact, continuity-corrected, or bootstrap approaches; the difference is therefore presented as an effect-size estimate. Error bars denote 95% confidence intervals for the group proportions. Site-level frequencies for the seven CpG sites are given in Supplementary Table S7.
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Frequency of FKBP5 intron 7 methylation positivity in adolescents with PTSD and trauma-unexposed controls. Methylation status was assessed qualitatively (methylation detected vs. not detected) using Sanger sequencing of bisulfite-converted <t>PCR</t> products targeting the FKBP5 intron 7 region. Methylation positivity was detected in 10 of 36 adolescents with PTSD (27.8%) and in 3 of 36 trauma-unexposed controls (8.3%). The panel reports the relative risk with its bias-corrected and accelerated bootstrap interval and the two-sided Fisher’s exact p value (RR = 3.33, 95% BCa CI [0.96, 12.40]; Haldane-Anscombe corrected RR = 3.00; Fisher’s exact p = .063). Because expected cell counts are small, the contrast reaches the conventional threshold under the uncorrected Pearson χ ² test ( p = .032) but not under Fisher’s exact, continuity-corrected, or bootstrap approaches; the difference is therefore presented as an effect-size estimate. Error bars denote 95% confidence intervals for the group proportions. Site-level frequencies for the seven CpG sites are given in Supplementary Table S7.
Real Time Pcr Amplification, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pcr amplification instrument  (Eppendorf AG)
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Frequency of FKBP5 intron 7 methylation positivity in adolescents with PTSD and trauma-unexposed controls. Methylation status was assessed qualitatively (methylation detected vs. not detected) using Sanger sequencing of bisulfite-converted <t>PCR</t> products targeting the FKBP5 intron 7 region. Methylation positivity was detected in 10 of 36 adolescents with PTSD (27.8%) and in 3 of 36 trauma-unexposed controls (8.3%). The panel reports the relative risk with its bias-corrected and accelerated bootstrap interval and the two-sided Fisher’s exact p value (RR = 3.33, 95% BCa CI [0.96, 12.40]; Haldane-Anscombe corrected RR = 3.00; Fisher’s exact p = .063). Because expected cell counts are small, the contrast reaches the conventional threshold under the uncorrected Pearson χ ² test ( p = .032) but not under Fisher’s exact, continuity-corrected, or bootstrap approaches; the difference is therefore presented as an effect-size estimate. Error bars denote 95% confidence intervals for the group proportions. Site-level frequencies for the seven CpG sites are given in Supplementary Table S7.
Pcr Amplification Instrument, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Frequency of FKBP5 intron 7 methylation positivity in adolescents with PTSD and trauma-unexposed controls. Methylation status was assessed qualitatively (methylation detected vs. not detected) using Sanger sequencing of bisulfite-converted PCR products targeting the FKBP5 intron 7 region. Methylation positivity was detected in 10 of 36 adolescents with PTSD (27.8%) and in 3 of 36 trauma-unexposed controls (8.3%). The panel reports the relative risk with its bias-corrected and accelerated bootstrap interval and the two-sided Fisher’s exact p value (RR = 3.33, 95% BCa CI [0.96, 12.40]; Haldane-Anscombe corrected RR = 3.00; Fisher’s exact p = .063). Because expected cell counts are small, the contrast reaches the conventional threshold under the uncorrected Pearson χ ² test ( p = .032) but not under Fisher’s exact, continuity-corrected, or bootstrap approaches; the difference is therefore presented as an effect-size estimate. Error bars denote 95% confidence intervals for the group proportions. Site-level frequencies for the seven CpG sites are given in Supplementary Table S7.

Journal: European Journal of Psychotraumatology

Article Title: FKBP5 methylation, temperament, and chronotype in adolescent posttraumatic stress disorder: a preliminary case–control study

doi: 10.1080/20008066.2026.2726603

Figure Lengend Snippet: Frequency of FKBP5 intron 7 methylation positivity in adolescents with PTSD and trauma-unexposed controls. Methylation status was assessed qualitatively (methylation detected vs. not detected) using Sanger sequencing of bisulfite-converted PCR products targeting the FKBP5 intron 7 region. Methylation positivity was detected in 10 of 36 adolescents with PTSD (27.8%) and in 3 of 36 trauma-unexposed controls (8.3%). The panel reports the relative risk with its bias-corrected and accelerated bootstrap interval and the two-sided Fisher’s exact p value (RR = 3.33, 95% BCa CI [0.96, 12.40]; Haldane-Anscombe corrected RR = 3.00; Fisher’s exact p = .063). Because expected cell counts are small, the contrast reaches the conventional threshold under the uncorrected Pearson χ ² test ( p = .032) but not under Fisher’s exact, continuity-corrected, or bootstrap approaches; the difference is therefore presented as an effect-size estimate. Error bars denote 95% confidence intervals for the group proportions. Site-level frequencies for the seven CpG sites are given in Supplementary Table S7.

Article Snippet: PCR amplification used HotStarTaq DNA Polymerase (Qiagen).

Techniques: Methylation, DNA Sequencing